Table 1. Expression of the MCRs, POMC, and prohormone convertases in primary human macrovascular endothelial cells. HAoEC: human aortic endothelial cells; HCAEC: human coronary artery endothelial cells; HA: human astrocytes (positive control). Three different primary lines for each type of EC were analysed by RT-qPCR: from ^c1,c4ECACC, ^c2,c5Lonza, and ^c3,c6Promocell. In the upper panel, detection levels are reported as: -, undetected; +, <35 Ct; and ++, <30 Ct. In the lower panel, MC1R expression levels in each cell line are shown as means ± SEM of triplicate technical replicates